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Zap-70(Ab-319) Antibody

21173
Review: comment rank 5 pdf
  Catalog No. Package Size Price
21173-1 50ul $189.00
21173-2 100ul $279.00

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Description
  • Product Name Zap-70(Ab-319) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Applications WB IHC
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of total Zap-70 protein.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around aa.317~321 (S-P-Y-S-D) derived from Human ZAP70.
  • Target Name Zap-70
  • Alternative Names 70 kDa zeta-associated protein; SRK; Syk-related tyrosine kinase; ZA70; ZAP-70
  • Accession No. Swiss-Prot: P43403
    NCBI Protein: NP _001070.2
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 70kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images
  • Zap-70(Ab-319) Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from HL60 cells using Zap-70(Ab-319) Antibody #21173.

  • Zap-70(Ab-319) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Zap-70(Ab-319) Antibody #21173(left) or the same antibody preincubated with blocking peptide(right).

Background

ZAP70 is a 70-kD tyrosine phosphoprotein that associates with the zeta chain and undergoes tyrosine phosphorylation following TCR stimulation. The ZAP70 gene is expressed in T- and natural KILLER cells. Protein-Tyrosine Kinases (PTKs) play an integral role in T-cell activation. Stimulation of the T-cell antigen receptor results in tyrosine phosphorylation of a number of cellular substrates. One of these is the TCR-zeta chain, which can mediate the transduction of extracellular stimuli into cellular effector functions

Salomon AR, et al. (2003) Proc Natl Acad Sci U S A; 100(2): 443-448
Ku GM, et al. (2001) EMBO J; 20(3): 457-465
Tang J, et al. (1999) Proc Natl Acad Sci U S A; 96(17): 9775-9780
Zhao Q, et al. (1996) Mol Cell Biol; 16(12): 6765-6774
Williams BL, et al. (1999) EMBO J; 18(7): 1832-1844
Adjali O, et al. (2005) J Clin Invest; 115(8): 2287-2295.

    Please let us know if you have published research using #21173 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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