• Product NameHP1-gamma Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.5G10-F7-A12
• IsotypeIgG1
• PurificationAffinity purified
• ApplicationsWB ICC IP IHC
• Species ReactivityMs Hu Mk Hm Rt
• SpecificityThis antibody detects endogenous levels of HP1-gamma and does not cross-react with related proteins.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionPurified recombinant human HP1-gamma protein fragments expressed in E.coli.
• Target NameHP1-gamma
• Alternative Names    CBX 3; CBX3; CBX3_HUMAN; Chromobox homolog 3 (HP1 gamma homolog, Drosophila); Chromobox homolog 3; Chromobox protein homolog 3; GAMMA; HECH; Heterochromatin like protein 1; Heterochromatin protein 1 homolog gamma; Heterochromatin protein HP1 gamma
• Accession No. Uniprot: Q13185

  Gene ID: 11335

• UniprotQ13185
• Gene ID11335;
• SDS-PAGE MW 22kd
• Formulation Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
• Storage store at -20˚C

• Western blotting: 1:1000

  Immunocytochemistry: 1:200

 Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

• Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-HP1-gamma antibody (dilution 1:200).
• Western blot detection of HP1-gamma in Hela,3T3,C6,COS7 and CHO-K1 cell lysates using HP1-gamma antibody (1:1000 diluted).Predicted band size:22KDa.Observed band size:22KDa.
• Immunoprecipitation analysis of Hela cell lysates using HP1-gamma antibody.
• Immunohistochemical analysis of paraffin-embedded Prostate Cancer using HP1-gamma Mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting