• Product NameKeratin 18 Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.2B5-B6-F7
• IsotypeIgG2a
• PurificationAffinity purified
• ApplicationsWB ICC
• Species ReactivityHu Mk
• SpecificityThis antibody detects endogenous levels of krt18 and does not cross-react with related proteins.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionPurified recombinant human KRT18 protein fragments expressed in E.coli.
• Target NameKeratin 18
• Alternative Names    Cell proliferation-inducing gene 46 protein; Cytokeratin-18; Keratin-18; krt18;
• Accession No. Uniprot: P05783

  Gene ID: 3875

• UniprotP05783
• Gene ID3875;
• SDS-PAGE MW 46kd
• Formulation Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide,0.1mg/mlBSA and 50% glycerol.
• Storage store at -20˚C

• Western blotting: 1:2000

  Immunocytochemistry: 1:800

 Involved in the uptake of thrombin-antithrombin complexes by hepatic cells By similarity. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

• Immunocytochemistry staining of HeLa cells fixed by anhydrous methanol for 2 h at -20℃ and using anti-krt18 antibody (dilution 1:800).
• Western blot detection of krt18 antibody in Hela,COS7 and 293T cell lysates using krt18 antibody (1:2000 diluted).Predicted band size:46KDa.Observed band size:46KDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting