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DNA-PKcs Monoclonal Antibody

27182
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  Catalog No. Package Size Price
27182-1 50ul $189.00
27182-2 100ul $279.00

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Description
  • Product Name DNA-PKcs Monoclonal Antibody
  • Host Species Mouse
  • Clonality Monoclonal
  • Clone No. 8D3-B12-F11
  • Isotype lgG2b
  • Purification Affinity purified
  • Applications WB IP ICC
  • Species Reactivity Hu
  • Specificity This antibody detects endogenous levels of DNA-PKcs,and does not cross-react with related proteins.
  • Immunogen Type Recombinant Protein
  • Immunogen Description Purified recombinant human DNA-PKcs protein fragments expressed in E.coli
  • Target Name DNA-PKcs
  • Alternative Names DNA dependent protein kinase catalytic subuni; DNA PKcs; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; DNAPK; DNPK1; hyper radiosensitivity of murine scid mutation, complementing 1; HYRC; HYRC1; p350; p460; PRKDC;
  • Accession No. Uniprot: P78527

    Gene ID: 5591

  • SDS-PAGE MW 450kd
  • Formulation Purified mouse monoclonal in PBS(pH 7.4)containing with 0.2% sodium azide,50% glycerol.
  • Storage store at -20˚C
Application Details

Western blotting: 1:1000

Immunocytochemistry: 1:200

Images
  • DNA-PKcs Monoclonal Antibody - SAB | Signalway Antibody

    Immunocytochemistry stain of Hela using PRKDC antibody (1:200).

  • DNA-PKcs Monoclonal Antibody - SAB | Signalway Antibody

    Immunoprecipitation analysis of Hela cell lysates using PRKDC antibody

  • DNA-PKcs Monoclonal Antibody - SAB | Signalway Antibody

    Western blot detection of PRKDC in Hela,K562,HL-60&MOLT-4 cell lysates using PRKDC antibody(1:1000 diluted).Predicted band size:450KDa,Observed band size:450KDa

  • DNA-PKcs Monoclonal Antibody - SAB | Signalway Antibody

    Western blot detection of PRKDC in K562,Molt-4,A549 and HCT116 cell lysates using PRKDC antibody(1:1000 diluted).Predicted band size:450KDa.Observed band size:450KDa.

Background

 properties.Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis(DCLRE1C).The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step.Required to protect and align broken ends of DNA.May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage.Found at the ends of chromosomes,suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion.Also involved in modulation of transcription.Recognizes the substrate consensus sequence[ST]-Q.Phosphorylates'Ser-139' of histone variant H2AX/H2AFX,thereby regulating DNA damage response mechanism.Phosphorylates DCLRE1C,c-Abl/ABL1,histone H1,HSPCA,c-jun/JUN,p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2.Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA.Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D.

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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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