• Product NameSmad2 Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.6H5-E3-C11
• IsotypeIgG1
• ApplicationsWB ICC
• Species ReactivityHu
• SpecificityThis antibody detects endogenous levels of SMAD2 and does not cross-react with related proteins.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionPurified recombinant human SMAD2 protein fragments expressed in E.coli.
• Target NameSmad2
• Alternative Names    hMAD 2; hMAD-2; hSMAD2; JV18 1; JV18; JV18; JV18-1; JV181; MAD; MAD; MAD homolog 2; MAD Related Protein 2; Mad-related protein 2; MADH2; MADR2; MGC22139; MGC34440; Mothers Against Decapentaplegic Homolog 2; Mothers Against Decapentaplegic Homolog 2;
• Accession No. Uniprot: Q15796

  Gene ID: 4087

• UniprotQ15796
• Gene ID4087;
• SDS-PAGE MW 60kd
• Formulation ascites
• Storage store at -20˚C

• Western blotting: 1:500

  Immunocytochemistry: 1:100

 Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases.Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and,on formation of the SMAD2/SMAD4 complex,activates transcription.May act as a tumor suppressor in colorectal carcinoma.Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

• Immunocytochemistry staining of HeLa cells fixed with 1% Paraformaldehyde and using anti-SMAD2 antibody (dilution 1:100).
• Western blot detection of SMAD2 antibody in Hela,A431,Jurkat and K562 cell lysates using SMAD2 antibody (1:500 diluted).Predicted band size:60KDa.Observed band size:60KDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting