• Product NameProminin-1 Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.6H10-F1-C11
• IsotypeIgG2a
• PurificationAffinity purified
• ApplicationsWB
• Species ReactivityHu
• SpecificityThis antibody detects endogenous levels of PROM1 and does not cross-react with related proteins.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionPurified recombinant human PROM1 protein fragments expressed in E.coli.
• Target NameProminin-1
• Accession No. Uniprot: O43490

  Gene ID: 8842

• UniprotO43490
• Gene ID8842;
• SDS-PAGE MW 133kd
• Formulation Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide,0.1mg/mlBSA and 50% glycerol.
• Storage store at -20˚C

•  Western blotting: 1:1000

 Binds cholesterol in cholesterol-containing plasma membrane microdomains.Proposed to play a role in apical plasma membrane organization of epithelial cells.During early retinal development acts as a key regulator of disk morphogenesis.Involved in regulation of MAPK and Akt signaling pathways.In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner.

• Western blot detection of PROM1 antibody in CaCo2 cell lysate using PROM1 antibody (1:1000 diluted).Predicted band size:97KDa.Observed band size:133KDa
• Western blot detection of PROM1 expression in CHO-k1 cells non-transfected (A) or transfected (B) with PROM1 and using PROM1 antibody (1:1000 diluted).Predicted band size:97KDa.Observed band size:133KDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting