• Product NameEstrogen Receptor- beta (Phospho-Ser105) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB;IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of Estrogen Receptor- beta only when phosphorylated at serine 105.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionThe antiserum was produced against synthesized peptide derived from human Estrogen Receptor-beta around the phosphorylation site of Ser105.
• Target NameEstrogen Receptor- beta
• ModificationPhospho
• Alternative Names    ESR2; ESTRB; Estrogen receptor beta; NR3A2;
• Accession No. Swiss-Prot#: Q92731;
  NCBI Gene#: 2100;
  NCBI Protein#: NP_001035365.1
• UniprotQ92731
• Gene ID2100;
• SDS-PAGE MW 59kd
• Concentration 1.0mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage Store at -20˚C/1 year

• WB 1:500 - 1:2000;
  IF 1:200 - 1:1000

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.

Hamilton-Burke W, et al. (2010)Am J Pathol 177, 1079-86. St-Laurent V, Sanchez M, Charbonneau C, Tremblay A (2005) J Steroid Biochem Mol Biol 94, 23-37. Tremblay A, Tremblay GB, Labrie F, Gigu¨¨re V (1999) Mol Cell 3, 513-9 .

• Western blot analysis of lysates from HeLa cells, using Estrogen Receptor-beta (Phospho-Ser105) Antibody. The lane on the right is blocked with the phospho peptide.
• Western Blot analysis of HepG2 cells using Estrogen Receptor-beta (Phospho-Ser105) Antibody
• Immunofluorescence analysis of HUVEC cells, using Estrogen Receptor-beta (Phospho-Ser105) Antibody. The picture on the right is blocked with the phospho peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting