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Ephrin-B2(Ab-330) Antibody

21196
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  Catalog No. Package Size Price
21196-1 50ul $189.00
21196-2 100ul $279.00

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Description
  • Product Name Ephrin-B2(Ab-330) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Applications WB IF
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of total Ephrin-B2 protein.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around aa.328~332 (N-I-Y-Y-K) derived from Human Ephrin B (ephrin-B2).
  • Target Name Ephrin-B2
  • Alternative Names EFNB2; HTKL; LERK5
  • Accession No. Swiss-Prot: P52799
    NCBI Protein: NP_004084.1
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 37kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Images
  • Ephrin-B2(Ab-330) Antibody - SAB | Signalway Antibody

    All lanes : Ephrin-B2(Ab-330) Antibody(21196) at 1/1000 dilutionLane 1 : Mouse spleen tissue; Lane 2 : Rat spleen tissue;Lane 3 : HEK293 cell extract;Lane 4 : 293T cell extract;Lysates/proteins at 40 µg per lane.Predicted band size : 37 kDaObserved band size : 37 kDa

  • Ephrin-B2(Ab-330) Antibody - SAB | Signalway Antibody

    21196 at 1/200 staining human renal clear cell carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

  • Ephrin-B2(Ab-330) Antibody - SAB | Signalway Antibody

    21196 at 1/200 staining human meningeal carcinomatosis(MC) tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary

  • Ephrin-B2(Ab-330) Antibody - SAB | Signalway Antibody

    21196 at 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary

  • Ephrin-B2(Ab-330) Antibody - SAB | Signalway Antibody

    21196 at 1/100 staining rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

  • Ephrin-B2(Ab-330) Antibody - SAB | Signalway Antibody

    Immunofluorescent analysis of formalin-fixed, paraffin-embedded hela cells with 21196 at 1/75 dilution. A Goat Anti-Rabbit IgG H&L (CY3) at 1/50 was used as secondary.

  • Ephrin-B2(Ab-330) Antibody - SAB | Signalway Antibody

    Immunofluorescent analysis of formalin-fixed, paraffin-embedded C6 cells with 21196 at 1/75 dilution. A Goat Anti-Rabbit IgG H&L (CY3) at 1/50 was used as secondary.

Background

Ephrin-B2 encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a lycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors.

Chrencik JE,et al.( 2006) J Biol Chem;281(38):28185-28192.
Kertesz N, et al. (2006) Blood; 107(6):2330-2338.
Noren NK, et al. (2004) Proc Natl Acad Sci USA; 101(15):5583-5588.

    Please let us know if you have published research using #21196 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
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    • ChHm Chinese Hamster 
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