• Product Namep90 RSK (Phospho-Thr573) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of P90RSK only when phosphorylated at threonine 573.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 573(L-M-T(p)-P-C) derived from Human p90 RSK.
• Target Namep90 RSK
• ModificationPhospho
• Alternative Names    KS6A1; p90RSK1; RSK1; RPS6KA1;
• Accession No. Swiss-Prot#: Q15418;
  NCBI Gene#: 6195;
  NCBI Protein#: NP_002944.2.
• UniprotQ15418
• Gene ID6195;
• SDS-PAGE MW 95kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100

Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes.

Eiichi Takahashi, J. Biol. Chem., Jul 1999; 274: 20206.
Ye Zhang, J. Biol. Chem., May 2002; 277: 19042 - 19048.
Ganesh R. Panta, Mol. Cell. Biol., Mar 2004; 24: 1823 - 1835.

• Western blot analysis of extracts from 293 cells treated with UV using p90 RSK (Phospho-Thr573) Antibody #11665.The lane on the right is treated with the antigen-specific peptide.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p90 RSK (Phospho-Thr573) antibody #11665 (left)or the same antibody preincubated with blocking peptide (right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting