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TUBA1/3/4 (Phospho-Tyr272) Antibody

11829
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  Catalog No. Package Size Price
11829-1 50ul $189.00
11829-2 100ul $279.00

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Description
  • Product Name TUBA1/3/4 (Phospho-Tyr272) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous levels of TUBA1/3/4 only when phosphorylated at tyrosine 272.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of tyrosine 272 (A-T-Y(p)-A-P) derived from Human TUBA1/3/4.
  • Target Name TUBA1/3/4
  • Modification Phospho
  • Alternative Names TBA1; TBA1A; TBA4A; TUBA1;
  • Accession No. Swiss-Prot#: Q71U36/P68363/Q9BQE3/Q13748/Q6PEY2/P68366;
    NCBI Gene#: 7846/10376/84790/113457/7278/112714/7277;
    NCBI Protein#: NP_001257328.1.
  • SDS-PAGE MW 50-55kd
  • Concentration 1.0mg/ml
  • Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20˚C/1 year
Application Details

Western blotting: 1:500~1:1000

Images
  • TUBA1/3/4 (Phospho-Tyr272) Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from Rat brain cells using TUBA1/3/4 (Phospho-Tyr272) Antibody #11829.The lane on the right is treated with the antigen-specific peptide.

Background

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species.

Adler A.J., Bioorg. Med. Chem. 9:1967-1976(2001).
Sugano S., Nat. Genet. 36:40-45(2004).
Venter J.C., Submitted (JUL-2005).

    Please let us know if you have published research using #11829 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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