• Product NameLC3 Antibody HRP Conjugated
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationPurified by Protein A.
• ApplicationsWB IHC-P IHC-F ICC
• Species ReactivityHu Ms Rt
• Immunogen DescriptionKLH conjugated synthetic peptide aa 27-59 121 derived from human LC3
• ConjugatesHRP
• Excitation EmissionN A
• Target NameLC3
• Alternative Names    LC3A: Microtubule-associated proteins 1A 1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A MAP1B light chain 3 A, MAP1A MAP1B LC3 A, Microtubule-associated protein 1 light chain 3 alpha Microtubule-associated
• Accession No. Swiss-Prot#Q9H492, Q9GZQ8
  NCBI Gene ID84557, 81631
• UniprotQ9H492
• Gene ID84557;
• Concentration 1mg ml
• Formulation 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• Storage Shipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.

• WB=1:500-2000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting