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Raf1(Phospho-Ser259) Antibody #11006

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Catalog No. Package Size Price Quantity
50ul $219.00
100ul $319.00
Availability:Yes
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Description
  • Product NameRaf1(Phospho-Ser259) Antibody
  • Host SpeciesRabbit
  • ClonalityPolyclonal
  • PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • ApplicationsWB IHC
  • Species ReactivityHu Ms Rt
  • SpecificityThe antibody detects endogenous level of Raf-1 only when phosphorylated at serine 259.
  • Immunogen TypePeptide-KLH
  • Immunogen DescriptionPeptide sequence around phosphorylation site of serine 259 (S-T-S(p)-T-P) derived from Human Raf1.
  • Target NameRaf1
  • ModificationPhospho
  • Alternative Names    C-RAF; C-Raf; CRAF
  • Accession No. Swiss-Prot: P04049
    NCBI Protein: NP _002871.1
  • UniprotP04049
  • Gene ID5894;
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Background

Published Papers

Baicalin induces cellular senescence in human colon cancer cells via upregulation of DEPP and the activation of Ras/Raf/MEK/ERK signaling.Incell Death Dis. On 2018 Feb 13 by Wang Z, Ma L et al..PMID:29440765, , (2018),
PMID: 29440765
Mycoplasma ovipneumoniae induces sheep airway epithelial cell apoptosis through an ERK signalling-mediated mitochondria pathway. In BMC Microbiol on 2016 Sep 23 by Yanan Li, Zhongjia Jiang,et al..PMID:27663303, , (2016),
PMID: 27663303
MicroRNA-146b, a sensitive indicator of mesenchymal stem cell repair of acute renal injury. In Stem Cells Transl Med on 2016 Oct by Yuan Zhu, Jing Yu, et al..PMID: 27400799 , , (2016),
PMID: 27400799
Effect of Chaihushugan San on Expression of the Raf/mitogen-activated Protein Kinase/Extracellular Signal-Regulated Kinase Pathway in the Hippocampi of Perimenopausal Rats Induced by Immobilization Stress .In J Tradit Chin Med on 2015 Aug by Li Shengqiang, Liang Wenna et al..PMID:26427116, , (2015),
PMID: 26427116
View All Published Papers Exosomes Derived From Human Mesenchymal Stem Cells Confer Drug Resistance in Gastric Cancer.In Cell Cycle on 2015 Aug 3 by Runbi Ji , Bin Zhang et al..PMID:26091251, , (2015),
PMID: 26091251
Prognostic Impact of Raf-1 and p-Raf-1 Expressions for Poor Survival Rate in Non-Small Cell Lung Cancer.In Cancer Sci on 2012 Oct by Zhi-Xin Qiu, Lei Wang,et al..PMID:22738312, , (2012),
PMID: 22738312
Prognostic Impact of Raf-1 and p-Raf-1 Expressions for Poor Survival Rate in Non-Small Cell Lung Cancer.In Cancer Sci on 2012 Oct by Zhi-Xin Qiu, Lei Wang,et al..PMID:22738312, , (2012),
PMID: 22738312
Please let us know if you have published research using #11006 so that we can cite your reference.

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Application

WBWestern Blotting

IHCImmunohistochemistry

IFImmunofluorescence

ICCImmunocytochemistry

FCFlow Cytometry

IPImmunoprecipitation

EELISA

DBDot Blotting

ChIPChromatin Immunoprecipitation

GICAGold Immunochromatography Assay

NCNegative Control

Species Reactivity

HuHuman

MsMouse 

RtRat 

Dm Drosophila melanogaster

C Caenorhabditis elegans

MkMonkey

RbRabbit

B Bovine 

D Dog

PPig

HmHamster

ChHm Chinese Hamster 

ChkChicken  

ShpSheep