• Product NameP38 MAPK(Ab-182) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total P38MAPK protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 180~184 (T-G-Y-V-A) derived from Human P38 MAPK.
• Target NameP38 MAPK
• Alternative Names    MAPK2; MAPKAPK-2; MAPKAPK2
• Accession No. Swiss-Prot: Q16539
  NCBI Protein: NP _001306.1
• UniprotQ16539
• Gene ID1432;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 43kd
  Western blotting: 1:500~1:1000
  

Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.

Ming Zheng, et al.(2005) The FASEB Journal. 19: 109-111
Bernt van den et al.(2001) Blink Immunology, 166: 582-587
Arshad Rahman, et al. (2004) Am J Physiol Lung Cell Mol Physiol 287: L1017-L1024
Osamu Yoshino, et al. (2003) Endocrinology & Metabolism Vol. 88: 2236-2241

• Western blot analysis of extracts from 293 and Hela cells using P38 MAPK(Ab-182) Antibody #21245.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting