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SFTPC Antibody

32459
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32459 100ul $279.00

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Description
  • Product Name SFTPC Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were purified by affinity purification using immunogen.
  • Applications WB IHC IF
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of total SFTPC protein.
  • Immunogen Type Recombinant Protein
  • Immunogen Description Recombinant protein of human SFTPC.
  • Target Name SFTPC
  • Alternative Names SP-C; PSP-C; SFTP2; SMDP2; BRICD6
  • Accession No. Swiss-Prot:P11686
    NCBI Gene ID:6440
  • SDS-PAGE MW 21KD
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20˚C
Application Details

Western blotting: 1:500 - 1:2000
Immunohistochemistry: 1:50 - 1:100
Immunofluorescence: 1:50 - 1:200

Images
  • SFTPC Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts of various cell lines, using SFTPC antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.

  • SFTPC Antibody - SAB | Signalway Antibody

    Immunohistochemistry of paraffin-embedded human lung using SFTPC antibody at dilution of 1:200 (40x lens).

  • SFTPC Antibody - SAB | Signalway Antibody

    Immunohistochemistry of paraffin-embedded rat lung using SFTPC antibody at dilution of 1:200 (40x lens).

  • SFTPC Antibody - SAB | Signalway Antibody

    Immunohistochemistry of paraffin-embedded mouse lung using SFTPC antibody at dilution of 1:200 (40x lens).

  • SFTPC Antibody - SAB | Signalway Antibody

    Immunofluorescence analysis of human lung cancer using SFTPC Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

  • SFTPC Antibody - SAB | Signalway Antibody

    Immunofluorescence analysis of mouse lung using SFTPC Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Background

This gene encodes the pulmonary-associated surfactant protein C (SPC), an extremely hydrophobic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 2, also called pulmonary alveolar proteinosis due to surfactant protein C deficiency, and are associated with interstitial lung disease in older infants, children, and adults. Alternatively spliced transcript variants encoding different protein isoforms have been identified.

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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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