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SHP-1(Ab-536) Antibody

21318
Review: comment rank 5 pdf
  Catalog No. Package Size Price
21318-1 50ul $189.00
21318-2 100ul $279.00

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Description
  • Product Name SHP-1(Ab-536) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Applications WB IHC IF
  • Species Reactivity Hu Ms
  • Specificity The antibody detects endogenous level of total SHP-1 protein.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around aa.534~538 (S-E-Y-G-N) derived from Human SHP-1.
  • Target Name SHP-1
  • Alternative Names 70Z-SHP; EC 3.1.3.48; HCP; HCPH; Hematopoietic cell protein-tyrosine phosphatase
  • Accession No. Swiss-Prot: P29350
    NCBI Protein: NP_002822.2
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 68kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images
  • SHP-1(Ab-536) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SHP-1(Ab-536) Antibody #21318(left) or the same antibody preincubated with blocking peptide(right).

  • SHP-1(Ab-536) Antibody - SAB | Signalway Antibody

    Immunofluorescence staining of methanol-fixed MCF cells using SHP-1(Ab-536) Antibody #21318.

Background

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.

Migone TS, et al. (1998) Proc Natl Acad Sci USA; 95(7): 3845-3850.
Timms JF, et al.(1998) Mol Cell Biol; 18(7): 3838-3850.

    Please let us know if you have published research using #21318 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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