• Product NameNR5A2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total NR5A2 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human NR5A2.
• Target NameNR5A2
• Alternative Names    B1F; CPF; FTF; B1F2; LRH1
• Accession No. Swiss-Prot:O00482
  NCBI Gene ID:2494
• UniprotO00482
• Gene ID2494;
• SDS-PAGE MW 61KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IF 1:50 - 1:200

Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development.

• Immunofluorescence analysis of A549 cells using NR5A2 . Blue: DAPI for nuclear staining.
• Western blot analysis of extracts of various cell lines, using NR5A2 at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting