• Product NameHSP90B (Ab-254) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total HSP90B protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized non-phosphopeptide derived from human HSP90B around the phosphorylation site of serine 254 (V-G-S(p)-D-E).
• Target NameHSP90B
• Alternative Names    HS90B; HSP 84; HSP90-beta; HSP90AB1; HSPC2
• Accession No. Swiss-Prot: P08238
  NCBI Gene ID: 3326
• UniprotP08238
• Gene ID3326;
• SDS-PAGE MW 92kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.

Lothar Goretzki, AACR Meeting Abstracts, Apr 2006; 2006: 468.
Jean-Fran?ois Louvion, Mol. Biol. Cell, Nov 1998; 9: 3071.
Angelika Lueking, Mol. Cell. Proteomics, Dec 2003; 2: 1342.
Jason Fontana Circ, Res., May 2002; 90: 866.

• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP90B (Ab-254) antibody #33117.
• Immunofluorescence analysis of HeLa cells, treated with TNF-a (20nM, 15mins), using HSP90B (Ab-254) antibody #33117.
• Western blot analysis of extracts from Hela cells treated with TNF-α (20ng/ml, 30min), using HSP90B (Ab-254) antibody #33117.
• Western blot analysis of extracts from HeLa cells (Lane 2), CoLo cells (Lane 3), HepG2 cells (Lane 4) and 293 cells (Lane 5), using HSP90B (Ab-254) antiobdy #33117. The lane on the left is treated with synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting