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AKT1/AKT2/AKT3(Ab-315/316/312) Antibody

21501
Review: comment rank 5 pdf
  Catalog No. Package Size Price
21501-1 50ul $189.00
21501-2 100ul $279.00

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Description
  • Product Name AKT1/AKT2/AKT3(Ab-315/316/312) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Applications IHC
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of total AKT1/AKT2/AKT3 protein.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around aa.313~317/314~318/310~314 (P-E-Y-L-A) derived from Human AKT1/AKT2/AKT3.
  • Target Name AKT1/AKT2/AKT3
  • Alternative Names RAC-PK-alpha; Protein kinase B;
  • Accession No. Swiss-Prot: P31749 P31751 Q9Y243
    NCBI Protein: NP _001014431.1 NP _001617.1 NP _005456.1
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 60kd
Immunohistochemistry: 1:50~1:100

Images
  • AKT1/AKT2/AKT3(Ab-315/316/312) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1/AKT2/AKT3(Ab-315/316/312) Antibody #21501(left) or the same antibody preincubated with blocking peptide(right).

Background
General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. /General protein kinase capable of phosphorylating several known proteins. /IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.

Nelms K, et al. (1999) Annu Rev Immunol. 17:701-738.
Malabarba M G, et al. (1996) Biochem. J. 319:865-872.
Hou J, et al. (1994) Science. 265:1701-1706.
Quelle F W, et al. (1995) Mol Cell Biol. 15: 3336-3343.
Takeda K, et al. (1996) Nature. 380: 627-630.
    Please let us know if you have published research using #21501 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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