• Product NameGR (Ab-226) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total GR protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized non-phosphopeptide derived from human GR around the phosphorylation site of serine 226/234/246 (L-L-S-P-L).
• Target NameGR
• Alternative Names    GCR; GRL; GRL1; NR3C1; glucocorticoid receptor
• Accession No. Swiss-Prot: P04150
  NCBI Gene ID: 2908
• UniprotP04150
• Gene ID2908;
• SDS-PAGE MW 90kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic genes expression.

Kyung Jin Kwak, J. Exp. Bot., Nov 2005; 56: 3007 - 3016.
Maite de Llanos, Hum. Reprod., Jan 2005; 20: 216 - 220.
M. Lynch, Mol. Hum. Reprod., Jul 2005; 11: 507 - 512.
Valeriya P. Makarenkova, J. Immunol., Feb 2006; 176: 2085 - 2094.

• Immunohistochemical analysis of paraffin-embedded human brain tissue using GR (Ab-226) antibody #33132.
• Western blot analysis of extracts from Jurkat cells, treated with EGF (200ng/ml, 15mins), using GR (Ab-226) antibody #33132.
• Western blot analysis of extracts from JK cells (Lane 2) and K562 cells (Lane 3), using GR(Ab-226) antiobdy #33132. The lane on the left is treated with synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting