Your Position: Home > Research Area > AKT1(Ab-450) Antibody

AKT1(Ab-450) Antibody

21502
Review: comment rank 5 pdf
  Catalog No. Package Size Price
21502-1 50ul $189.00
21502-2 100ul $279.00

Quantity:  Total:   
 

Availability: promotionYes

add promotion
Description
  • Product Name AKT1(Ab-450) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Applications WB IHC IF
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of total AKT1 protein.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around aa.448~452 (T-I-T-P-P) derived from Human AKT1.
  • Target Name AKT1
  • Alternative Names RAC-PK-alpha; Protein kinase B;
  • Accession No. Swiss-Prot: P31749
    NCBI Protein: NP_001014431.1
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images
  • AKT1(Ab-450) Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from 293 and MCF cells using AKT1(Ab-450) Antibody #21502.

  • AKT1(Ab-450) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1(Ab-450) Antibody #21502(left) or the same antibody preincubated with blocking peptide(right).

  • AKT1(Ab-450) Antibody - SAB | Signalway Antibody

    Immunofluorescence staining of methanol-fixed Hela cells using AKT1(Ab-450) Antibody #21502.

Background
General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.

Xing J, et al. (1998) Mol Cell Biol 18(4): 1946-55.
Tan Y, et al.( 1996) EMBO J; 15(17): 4629-42.
Hao, M. et al. (1996) J. Biol. Chem. 271, 29380-29385.
Mayo LD, et al. (2001) Biol Chem; 276(27): 25184-9.
Lu, H. et al. (1997) Mol. Cell. Biol. 17, 5923-5934.
    Please let us know if you have published research using #21502 so that we can cite your reference.
Customer Reviews and Rankings
  • No comment
  • Total 0 results, divided into1 pages. First Prev Next Last

    My Review

    Username: Anonymous user
    E-mail:
    Ratings:
    Content:
    Verification code: captcha
     
    Protocol
    Browsing historyclear
    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
      SUBSCRIBE OUR E-MAIL
    • Inspiration, ideas and your feedback.
    FOLLOW US

    facebook twitter linkedin

    sab © 2019 Signalway Antibody LLC. All Rights Reserved.  E-mail: info@signalwayantibody.com