• Product NameMITF (Ab-180/73) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total MITF protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized non-phosphopeptide derived from human MITF around the phosphorylation site of serine 180/73 (P-N-S(p)-P-M).
• Target NameMITF
• Alternative Names    MITF M1; Microphthalmia-associated transcription factor;
• Accession No. Swiss-Prot: O75030
  NCBI Gene ID: 4286
• UniprotO75030
• Gene ID4286;
• SDS-PAGE MW 52kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

J¨®n H. Hallsson, Genetics, May 2004; 167: 233 - 241.
Eiichi Morii, Blood, Apr 2001; 97: 2038 - 2044.
Eiichi Morii, Blood, Sep 2004; 104: 1656 - 1661.

• Immunohistochemistry analysis of paraffin-embedded human skin tissue using MITF (Ab-180/73) antibody #33138.
• Immunofluorescence analysis of HeLa cells, using MITF (Ab-180/73) antibody #33138.
• Western blot analysis of extracts from HepG2 cells (lane 1) and COLO205 cells (lane 2), using MITF (Ab-180/73) antibody #33138.
• Western blot analysis of extracts from HepG2 cells (Lane 2) and 3T3 cells (Lane 3), using MITF (Ab-180/73) antiobdy #33138. The lane on the left is treated with synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting