• Product NameSF1 (Ab-82) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total SF1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized non-phosphopeptide derived from human SF1 around the phosphorylation site of serine 82 (S-P-S(p)-P-E).
• Target NameSF1
• Alternative Names    BBP; CW17; Mammalian branch point binding protein mBBP; SF01; Splicing factor 1
• Accession No. Swiss-Prot: Q15637
  NCBI Gene ID: 7536
• UniprotQ15637
• Gene ID7536;
• SDS-PAGE MW 68kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.

Susan Y. Park, Development, May 2005; 132: 2415 - 2423.
Dujin Zhou, Mol. Endocrinol., Jul 2000; 14: 986.
Dagmar Wilhelm, Genes & Dev., Jul 2002; 16: 1839.
Philipp Gut,Development, Oct 2005; 132: 4611 - 4619.

• Western blot analysis of extracts from COLO205 cells, using SF1 (Ab-82) antibody #33142.
• Immunohistochemistry analysis of paraffin-embedded human brain tissue using SF1 (Ab-82) antibody #33142.
• Immunofluorescence analysis of HeLa cells, using SF1 (Ab-82) antibody #33142.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting