• Product NameBax (Ab-167) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IF
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total Bax protein
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized non-phosphopeptide derived from human Bax around the phosphorylation site of threonine 167 (F-G-T(p)-P-T).
• Target NameBax
• Alternative Names    Apoptosis regulator BAX; membrane isoform alpha; BAXA; Bcl2-associated X protein;
• Accession No. Swiss-Prot: Q07812
  NCBI Gene ID: 581
• UniprotQ07812
• Gene ID581;
• SDS-PAGE MW 21kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  Immunofluorescence: 1:100~1:500

Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

Oltvai Z.N., Cell 74:609-619(1993).
Cuddeback S.M., J. Biol. Chem. 276:20559-20565(2001).
Guo B., Nature 423:456-461(2003)

• Western blot analysis of extracts from HepG2 cells, using Bax (Ab-167) antibody #33148.
• Immunofluorescence analysis of LOVO cells, using Bax (Ab-167) antibody #33148.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting