• Product NameSmad1 (Ab-187) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total Smad1 protein
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized non-phosphopeptide derived from human Smad1 around the phosphorylation site of serine 187 (P-H-S(p)-P-N).
• Target NameSmad1
• Alternative Names    BSP-1; BSP1; Dwarfin-A; Dwf-A; JV4-1
• Accession No. Swiss-Prot: Q15797
  NCBI Gene ID: 4086
• UniprotQ15797
• Gene ID4086;
• SDS-PAGE MW 60kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  Immunofluorescence: 1:100~1:500

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.

Riggins G.J., Nat. Genet. 13:347-349(1996).
Liu F., Nature 381:620-623(1996).
Hoodless P.A., Cell 85:489-500(1996)

• Western blot analysis of extracts from JK and K562 cells, using Smad1 (Ab-187) antibody #33163.
• Immunofluorescence analysis of HeLa cells, using Smad1 (Ab-187) antibody #33163.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting