• Product NamePI3 Kinase p85/p55(Ab-467/199) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsIHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total PI3 Kinase p85/p55 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.465~469(L-Y-E-E-Y) derived from Human PI3 Kinase p85/p55.
• Target NamePI3 Kinase p85/p55
• Alternative Names    p85, AGM7, p85-ALPHA, p55, p55-GAMMA
• Accession No. Swiss-Prot:P27986
  Gene ID:5295
• UniprotP27986
• Gene ID5295;
• SDS-PAGE MW 55, 85kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 55, 85kd
  Immunohistochemistry: 1:50~1:100

PI3K binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

Stoyanov, B. et al. (1995) Science 269, 690-3.
Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41.
Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80.

• Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PI3K P85 a/g/b(Ab-467/199/464) Antibody #21508(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting