• Product NameAurB/C (Ab-202/175) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total AurB/C protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized non-phosphopeptide derived from human AurB/C around the phosphorylation site of threonine 202/175 (C-G-T(p)-L-D).
• Target NameAurB/C
• Alternative Names    AIE1; AIE2; AURKC; Aurora-C; Aurora/Ipl1-related kinase 3
• Accession No. Swiss-Prot: Q9UQB9
  NCBI Gene ID: 9212/6795
• UniprotQ9UQB9
• Gene ID6795;
• SDS-PAGE MW 39kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Plays also a role in meiosis and more particularly in spermatogenesis. Has redundant cellular functions with AURKB and can rescue an AURKB knockdown. Like AURKB, AURKC phosphorylates histone H3 at 'Ser-10' and 'Ser-28'. Phosphorylates TACC1, another protein involved in cell division, at 'Ser-228'.

Tseng T.-C., DNA Cell Biol. 17:823-833(1998).
Bernard M., Genomics 53:406-409(1998).
Kimura M., J. Biol. Chem. 274:7334-7340(1999)

• Western blot analysis of extracts from 293, Hela and HepG2 cells, using AurB/C (Ab-202/175) antibody #33177.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting