• Product NameNeutrophil Cytosol Factor 1 (Ab-304) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total Neutrophil Cytosol Factor 1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized non-phosphopeptide derived from human Neutrophil Cytosol Factor 1 around the phosphorylation site of serine 304 (R-S-S(p)-I-R).
• Target NameNeutrophil Cytosol Factor 1
• Alternative Names    47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-1; NCF-47K; NCF1
• Accession No. Swiss-Prot: P14598
  NCBI Gene ID: 653361
• UniprotP14598
• Gene ID653361;
• SDS-PAGE MW 44kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  Immunofluorescence: 1:100~1:500

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

Lomax K.J., Science 245:409-412(1989).
Rodaway A.R.F., Mol. Cell. Biol. 10:5388-5396(1990).
Gu Y., Exp. Cell Res. 272:62-74(2002).

• Immunofluorescence analysis of HeLa cells, using Neutrophil Cytosol Factor 1 (Ab-304) antibody #33180.
• Western blot analysis of extracts from colo cells, using Neutrophil Cytosol Factor 1 (Ab-304) antibody #33180.
• Western blot analysis of extracts from HepG2 cells (Lane 2) and Jurket cells (Lane 3), using Neutrophil Cytosol Factor 1 (Ab-304) antiobdy #33180. The lane on the left is treated with synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting