• Product NameMAP2K3 (Ab-222) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total MAP2K3 protein
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized non-phosphopeptide derived from human MAP2K3 around the phosphorylation site of threonine 222 (A-K-T(p)-M-D).
• Target NameMAP2K3
• Alternative Names    dual specificity mitogen-activated protein kinase kinase 3; EC 2.7.12.2; kinase MKK3; MAP kinase kinase 3; MAP2K3
• Accession No. Swiss-Prot: P46734
  NCBI Gene ID: 5606
• UniprotP46734
• Gene ID5606;
• SDS-PAGE MW 39kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.

Derijard B.,Science 267:682-685(1995).
The MGC Project Team;Genome Res. 14:2121-2127(2004).
Lim S.,J. Biol. Chem. 277:25040-25046(2002).

• Western blot analysis of extracts from Jurkat cells, treated with serum (20%, 15mins), using MAP2K3 (Ab-222) antibody #33236.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting