• Product NameB-Raf (Ab-753) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Rt
• SpecificityThe antibody detects endogenous levels of total B-Raf protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized non-phosphopeptide derived from human B-Raf around the phosphorylation site of threonine 735 (P-K-T(p)-P-I).
• Target NameB-Raf
• Alternative Names    B-RAF; B-RAF proto-oncogene serine/threonine-protein kinase; B-Raf proto-oncogene serine/threonine-protein kinase; BRAF; BRAF1
• Accession No. Swiss-Prot: P15056
  NCBI Gene ID: 673
• UniprotP15056
• Gene ID673;
• SDS-PAGE MW 80kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

Stephens R.M., Mol. Cell. Biol. 12:3733-3742(1992).
Hillier L.W., Nature 424:157-164(2003).
Eychene A., Oncogene 7:1657-1660(1992).

• Western blot analysis of extracts from rat brain cells, using B-Raf (Ab-753) antibody #33277.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting