• Product NameGab2 (Ab-623) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total Gab2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized non-phosphopeptide derived from human Gab2 around the phosphorylation site of serine 623 (P-S-S(p)-P-S).
• Target NameGab2
• Alternative Names    GRB2-associated-binding protein 2; Growth factor receptor bound protein 2-associated protein 2; GRB2-associated binder 2; pp100; KIAA0571
• Accession No. Swiss-Prot: Q9UQC2
  NCBI Gene ID: 9846
• UniprotQ9UQC2
• Gene ID9846;
• SDS-PAGE MW 74kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Adapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis.

Nishida K., Blood 93:1809-1816(1999).
Nagase T., DNA Res. 5:31-39(1998).
Salomon A.R., Proc. Natl. Acad. Sci. U.S.A. 100:443-448(2003).

• Western blot analysis of extracts from Jurkat cells, treated with TNF (2500U/ml, 30mins), using Gab2 (Ab-623) antibody #33284.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting