• Product NameMnk1 (Ab-385) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total Mnk1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized non-phosphopeptide derived from human Mnk1 around the phosphorylation site of threonine 385 (L-P-T(p)-P-Q).
• Target NameMnk1
• Alternative Names    EC 2.7.11.1; kinase Mnk1; Map kinase interacting kinase; MAP kinase signal-integrating kinase 1; MAP kinase-interacting serine/threonine kinase 1
• Accession No. Swiss-Prot: Q9BUB5
  NCBI Gene ID: 8569
• UniprotQ9BUB5
• Gene ID8569;
• SDS-PAGE MW 45kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.

Fukunaga R., EMBO J. 16:1921-1933(1997).
Knauf U., Mol. Cell. Biol. 21:5500-5511(2001).
O'Loghlen A., Exp. Cell Res. 299:343-355(2004).

• Western blot analysis of extracts from 3T3 cells, treated with PMA (125ng/ml, 30mins), using Mnk1 (Ab-385) antibody #33288.
• Western blot analysis of extracts from HepG2 cells (Lane 2), K562 cells (Lane 3) and Jurket cells (Lane 4), using Mnk1 (Ab-385) antiobdy #33288. The lane on the left is treated with systhesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting