• Product NameTUBA1/3/4 (Ab-272) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Rt
• SpecificityThe antibody detects endogenous levels of total TUBA1/3/4 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized non-phosphopeptide derived from human TUBA1/3/4 around the phosphorylation site of tyrosine 272 (A-T-Y(p)-A-P).
• Target NameTUBA1/3/4
• Alternative Names    Alpha-tubulin 1; TBA1; TBA1A; TBA4A; Testis-specific alpha- tubulin
• Accession No. Swiss-Prot: Q71U36/P68363/Q9BQE3/Q13748/Q6PEY2/P68366
  NCBI Gene ID: 7846/10376/84790/113457/7278/112714/7277
• UniprotQ71U36
• Gene ID7846;
• SDS-PAGE MW 50-56kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Adler A.J., Bioorg. Med. Chem. 9:1967-1976(2001).
Sugano S., Nat. Genet. 36:40-45(2004).
Venter J.C., Submitted (JUL-2005).

• Western blot analysis of extracts from Rat brain cells, using TUBA1/3/4 (Ab-272) antibody #33306.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting