• Product NameBak Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total Bak protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from human Bak.
• Target NameBak
• Alternative Names    Bcl-2 homologous antagonist/killer; Apoptosis regulator BAK; Bcl-2-like 7 protein; BAK1; BAK
• Accession No. Swiss-Prot: Q16611
  NCBI Gene ID: 578
• UniprotQ16611
• Gene ID578;
• SDS-PAGE MW 25kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis.

Takuma Uo, J. Biol. Chem., Mar 2005; 280: 9065 - 9073.
Pierre-Fran?ois Cartron, Mol. Cell. Biol., Jul 2003; 23: 4701 - 4712.
Joslyn K. Brunelle, J. Biol. Chem., Feb 2004; 279: 4305 - 4312.
Neil A. Jones, Mol. Pharmacol., May 1998; 53: 819.

• Western blot analysis of extracts from 293 cells, using Bak antibody #33326.
• Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using Bak antibody #33326.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting