• Product NameClaudin 10 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total Claudin 10 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from human Claudin 10.
• Target NameClaudin 10
• Alternative Names    Claudin-10; OSP-like protein; CLDN10;
• Accession No. Swiss-Prot: P78369
  NCBI Gene ID: 9071
• UniprotP78369
• Gene ID9071;
• SDS-PAGE MW 25kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. May form permselective paracellular pores; isoform 1 appears to create pores preferentially permeable to cations and isoform 2 for anions. Plays a key role in controlling cation selectivity and transport in the thick ascending limb (TAL) of Henle's loop in kidney.

Siu Tim Cheung, Clin. Cancer Res., Jan 2005; 11: 551 - 556.
Hiroki Fujita, J. Histochem. Cytochem., Aug 2006; 54: 933 - 944.
Christina M. Van Itallie, Am J Physiol Renal Physiol, Dec 2006; 291: F1288 - F1299.
Anne Florin, Endocrinology, Mar 2005; 146: 153

• Western blot analysis of extracts from LOVO cells, using Claudin 10 antibody #33338.
• Western blot analysis of extracts from A549 cells (Lane 2), using Claudin 10 antiobdy #33338. The lane on the left is treated with synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting