• Product NameCREB-BP Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total CREB-BP protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from human CREB-BP.
• Target NameCREB-BP
• Alternative Names    CREB-binding protein; CBP CBP/MOZ FUSION GENE;
• Accession No. Swiss-Prot: Q92793
  NCBI Gene ID: 1387
• UniprotQ92793
• Gene ID1387;
• SDS-PAGE MW 265kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Acetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 and FOXO1. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1 in the presence of EP300. Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers.

Zoi Lygerou, Nucleic Acids Res., Feb 1994; 22: 5332 - 5340.
Karsten Grote, J. Biol. Chem., Dec 2004; 279: 55675 - 55681.
Kumi Nagamoto-Combs, J. Biol. Chem., Feb 1997; 272: 6051.
Stephen J. Elliman, Isaac, J. Biol. Chem., Jan 2006; 281: 16 -19

• Western blot analysis of extracts from HeLa cells, treated with insulin (0.01U/ml, 15mins), using CREB-BP antibody #33346.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB-BP antibody #33346.
• Immunofluorescence analysis of A549 cells, using CREB-BP antibody #33346.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting