• Product NameCullin 2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total Cullin 2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from human Cullin 2.
• Target NameCullin 2
• Alternative Names    Cullin-2; CUL-2;
• Accession No. Swiss-Prot: Q13617
  NCBI Gene ID: 8453
• UniprotQ13617
• Gene ID8453;
• SDS-PAGE MW 82,84kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 By similarity. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).

Seiji Shiraishi, J. Biol. Chem., Mar 2007; 282: 9017 - 9028.
Isabelle Groulx, J. Biol. Chem., Mar 2000; 275: 8991.
Dimitris Liakopoulos, PNAS, May 1999; 96: 5510.
Kyungwon Huh, AACR Meeting Abstracts, Apr 2006; 2006: 1326.

• Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Cullin 2 antibody #33348.
• Immunofluorescence analysis of NIH/3T3 cells, using Cullin 2 antibody #33348.
• Western blot analysis of extracts from LOVO cells, using Cullin 2 antibody #33348.
• Western blot analysis of extracts from HepG2 cells (Lane 2), using Cullin 2 antiobdy #33348. The lane on the left is treated with systhesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting