• Product NameCyclin F Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total Cyclin F protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from human Cyclin F.
• Target NameCyclin F
• Alternative Names    FBX1; FBXO1; F-box only protein 1;
• Accession No. Swiss-Prot: P41002
  NCBI Gene ID: 899
• UniprotP41002
• Gene ID899;
• SDS-PAGE MW 88kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of CP110 during G2 phase, thereby acting as an inhibitor of centrosome reduplication.

Tsz Kan Fung, J. Biol. Chem., Sep 2002; 277: 35140 - 35149.
Michael T. Tetzlaff, Mol. Cell. Biol., Mar 2004; 24: 2487 - 2498.
Cain H. Yam, Mol. Cell. Biol., Jan 1999; 19: 635 - 645.
Michael T. Tetzlaff, PNAS, Mar 2004; 101: 3338 - 3345.

• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin F antibody #33352.
• Western blot analysis of extracts from HeLa cells, using Cyclin F antibody #33352.
• Western blot analysis of extracts from HeLa cells (Lane 2), A549 cells (Lane 3) and HepG2 cells (Lane 4), using Cyclin F antiobdy #33352. The lane on the left is treated with systhesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting