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DNA-PK Antibody

33358
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  Catalog No. Package Size Price
33358-1 50ul $189.00
33358-2 100ul $279.00

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Description
  • Product Name DNA-PK Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Applications WB IHC IF
  • Species Reactivity Hu
  • Specificity The antibody detects endogenous levels of total DNA-PK protein.
  • Immunogen Type Peptide
  • Immunogen Description Synthesized peptide derived from human DNA-PK.
  • Target Name DNA-PK
  • Alternative Names DNA- PKcs; DNA-dependent protein kinase catalytic subunit; DNPK1; EC 2.7.11.1; P460
  • Accession No. Swiss-Prot: P78527
    NCBI Gene ID: 5591
  • SDS-PAGE MW 450kd
  • Concentration 1.0mg/ml
  • Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20˚C
Application Details

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Images
  • DNA-PK Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using DNA-PK antibody #33358.

  • DNA-PK Antibody - SAB | Signalway Antibody

    Immunofluorescence analysis of HeLa cells, treated with Forskolin (40nM, 30mins), using DNA-PK antibody #33358.

  • DNA-PK Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from HeLa cells using DNA-PK antibody #33358.

  • DNA-PK Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from HeLa cells (Lane 2), using DNA-PK antiobdy #33358. The lane on the left is treated with systhesized peptide.

Background
Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect machanism. Interacts with CRY1 and CRY2; negatively regulates CRY1 phosphorylation.

Wesley D. Block, Nucleic Acids Res., Apr 2004; 32: 1967 - 1972.
Katherine S. Pawelczak, Nucleic Acids Res., Jan 2005; 33: 152 - 161.
Eun-Jung Park, Nucleic Acids Res., Dec 2003; 31: 6819 - 6827.
Suisheng Zhang, Nucleic Acids Res., Jan 2004; 32: 1 - 10.
    Please let us know if you have published research using #33358 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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