• Product NameDP-1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total DP-1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from human DP-1.
• Target NameDP-1
• Alternative Names    E2F DIMERIZATION PARTNER 1; TRANSCRIPTION FACTOR DP1; TRANSCRIPTION FACTOR; SEQUENCE-SPECIFIC; DRTF1
• Accession No. Swiss-Prot: Q14186
  NCBI Gene ID: 7027
• UniprotQ14186
• Gene ID7027;
• SDS-PAGE MW 55kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DP2/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F1/DP complex appears to mediate both cell proliferation and apoptosis.

K Helin, Genes & Dev., Oct 1993; 7: 1850 - 1861.
B Shan, Cell Growth Differ., Jun 1996; 7: 689.
Hironori Ishida, J. Biol. Chem., Jul 2005; 280: 24642 - 24648.
Wing Y. Chang, J. Biol. Chem., Dec 2004; 279: 51343 - 51353.

• Western blot analysis of extracts from HeLa cells, using DP-1 antibody #33359.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using DP-1 antibody #33359.
• Immunofluorescence analysis of HeLa cells, treated with Forskolin (40nM, 30mins), using DP-1 antibody #33359.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting