• Product NameDysferlin Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total Dysferlin protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from human Dysferlin.
• Target NameDysferlin
• Alternative Names    dysferlin; limb girdle muscular dystrophy 2B (autosomal recessive);
• Accession No. Swiss-Prot: O75923
  NCBI Gene ID: 8291
• UniprotO75923
• Gene ID8291;
• SDS-PAGE MW 240kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Key calcium ion sensor involved in the Ca2+-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress By similarity.

Delia J. Hern¨¢ndez-Deviez, Hum. Mol. Genet., Jan 2006; 15: 129 - 142.
Yanchao Huang, FASEB J, Mar 2007; 21: 732 - 742.
Mengfatt Ho, Hum. Mol. Genet., Sep 2004; 13: 1999 - 2010.
Eriko Fujita, Hum. Mol. Genet., Mar 2007; 16: 618 - 629.

• Western blot analysis of extracts from K562 cells, using Dysferlin antibody #33360.
• Western blot analysis of extracts from JK cells (Lane 2) and K562 cells (Lane 3), using Dysferlin antiobdy #33360. The lane on the left is treated with synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting