• Product NameE2F4 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total E2F4 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from human E2F4.
• Target NameE2F4
• Alternative Names    E2F-4; p107/p130-binding protein;
• Accession No. Swiss-Prot: Q16254
  NCBI Gene ID: 1874/1875
• UniprotQ16254
• Gene ID1874;
• SDS-PAGE MW 44kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1.

Kathryn M. Kinross, Blood, Aug 2006; 108: 886 - 895.
Dawei Wang, Mol. Cell. Biol., May 2000; 20: 3417 - 3424.
Stephan P. Persengiev, Mol. Cell. Biol., Sep 1999; 19: 6048 - 6056.
Stefan Gaubatz, Mol. Cell. Biol., Feb 2001; 21: 1384 - 1392.

• Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using E2F4 antibody #33361.
• Western blot analysis of extracts from COS7 cells, using E2F4 antibody #33361.
• Western blot analysis of extracts from JK cells (Lane 2), using E2F4 antiobdy #33361. The lane on the left is treated with synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting