• Product NameFAS ligand Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total FAS ligand protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human FAS ligand.
• Target NameFAS ligand
• Alternative Names    Tumor necrosis factor ligand superfamily member 6; Fas antigen ligand; Fas ligand; CD178 antigen; CD95L protein
• Accession No. Swiss-Prot: P48023
  NCBI Gene ID: 356
• UniprotP48023
• Gene ID356;
• SDS-PAGE MW 33kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. The FasL intracellular domain (FasL ICD) cytoplasmic form induces gene transcription inhibition.

Alderson M.; J. Exp. Med. 181:71-77(1995).
Takahashi T., Int. Immunol. 6:1567-1574(1994).
Gregory S.G., ure 441:315-321(2006).

• Immunohistochemistry analysis of paraffin-embedded human brain tissue using FAS ligand antibody #33370.
• Western blot analysis of extracts from 293 cellls, using FAS ligand antibody #33370.
• Western blot analysis of extracts from 3T3 cells (Lane 2), using FAS ligand antiobdy #33370. The lane on the left is treated with synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting