• Product NameIntegrin β5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total Integrin β5 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from human Integrin β5.
• Target NameIntegrin β5
• Alternative Names    INTEGRIN BETA-5; ITGB5;
• Accession No. Swiss-Prot: P18084
  NCBI Gene ID: 3693
• UniprotP18084
• Gene ID3693;
• SDS-PAGE MW 88kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Integrin alpha-V/beta-5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand.

Hongquan Zhang, J. Cell Biol., Sep 2002; 158: 1287.
TJ Wickham, J. Cell Biol., Oct 1994; 127: 257.
BE Vogel, J. Cell Biol., Apr 1993; 121: 461.
JW Smith, J. Biol. Chem., Jul 1990; 265: 11008 - 11013.

• Western blot analysis of extracts from COS7 cells, using Integrin β5 antibody #33409.
• Western blot analysis of extracts from JK cells (Lane 2) and cos-7 cells (Lane 3), using Integrin β5 antiobdy #33409. The lane on the left is treated with synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting