• Product NameMMP-7 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total MMP-7 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from human MMP-7.
• Target NameMMP-7
• Alternative Names    PUMP1; PUTATIVE METALLOPROTEINASE I; matrix metalloproteinase 7 (matrilysin; uterine);
• Accession No. Swiss-Prot: P09237
  NCBI Gene ID: 4316
• UniprotP09237
• Gene ID4316;
• SDS-PAGE MW 29kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.

Lydia E. Wroblewski, J. Cell Sci., Jul 2003; 116: 3017.
Andrea Varro, Am J Physiol Gastrointest Liver Physiol, Apr 2007; 292: G1133 - G1140.
Wei-Hsuan Yu, Genes & Dev., Feb 2002; 16: 307.
Nick Di Girolamo, Invest. Ophthalmol. Vis. Sci., Aug 2001; 42: 1

• Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using MMP-7 antibody #33444.
• Western blot analysis of extracts from COS7 cells, using MMP-7 antibody #33444.
• Western blot analysis of extracts from COS7 cells (Lane 2) and HuvEc cells (Lane 3), using MMP-7 antiobdy #33444. The lane on the left is treated with systhesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting