• Product NameMMP-9 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total MMP-9 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from human MMP-9.
• Target NameMMP-9
• Alternative Names    92 kDa gelatinase; 92 kDa type IV collagenase precursor; CLG4B; GELB; Gelatinase B
• Accession No. Swiss-Prot: P14780
  NCBI Gene ID: 4318
• UniprotP14780
• Gene ID4318;
• SDS-PAGE MW 78kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.

Martin Illemann, Mol. Cancer Res., May 2006; 4: 293 - 302.
Noemi Ramos-DeSimone, J. Biol. Chem., May 1999; 274: 13066.
Vandana Iyer, J. Cell Sci., Mar 2005; 118: 1185 - 1195.
Jon R. Backstrom, J. Neurosci., Dec 1996; 16: 7910.

• Western blot analysis of extracts from HeLa cells, using MMP-9 antibody #33446.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MMP-9 antibody #33446.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting