• Product NameNCK2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsIHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total NCK2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from C-terminal of human NCK2.
• Target NameNCK2
• Alternative Names    Cytoplasmic protein NCK2; GRB4; NCK adaptor protein 2; NCKbeta; Nck-2
• Accession No. Swiss-Prot: O43639
  NCBI Gene ID: 8440
• UniprotO43639
• Gene ID8440;
• SDS-PAGE MW 43kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• 
  Immunohistochemistry: 1:50~1:100
  

Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.

Susanne Frese, J. Biol. Chem., Jun 2006; 281: 18236 - 18245.
Friedhelm Bladt, Mol. Cell. Biol., Jul 2003; 23: 4586 - 4597.
Stephan J. Kempiak, J. Biol. Chem., Feb 2005; 280: 5836 - 5842.
Min Chen, Mol. Cell. Biol., Nov 2000; 20: 7867 - 7880.

• Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using NCK2 antibody #33447.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting