• Product NameNKX3.1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsIHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total NKX3.1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from N-terminal of human NKX3.
• Target NameNKX3.1
• Alternative Names    Homeobox protein Nkx-3.1; NKX31; NKX3A;
• Accession No. Swiss-Prot: Q99801
  NCBI Gene ID: 4824
• UniprotQ99801
• Gene ID4824;
• SDS-PAGE MW 38kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• 
  Immunohistochemistry: 1:50~1:100
  

Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells.

Cai Bowen, Cancer Res., Jan 2007; 67: 455 - 464.
Xiang Li, Mol. Cell. Biol., Apr 2006; 26: 3008 - 3017.
Ekatherine Asatiani, Cancer Res., Feb 2005; 65: 1164 - 1173.
Carlise R. Bethel, Cancer Res., Nov 2006; 66: 10683 - 10690.

• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using NKX3.1 antibody #33448.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting