• Product Namep15 INK Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total p15 INK protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from human p15 INK antibody.
• Target Namep15 INK
• Alternative Names    CDKN2B; CDN2B; Cyclin-dependent kinase 4 inhibitor B; MTS2; Multiple tumor suppressor 2
• Accession No. Swiss-Prot: P42772
  NCBI Gene ID: 1030
• UniprotP42772
• Gene ID1030;
• SDS-PAGE MW 14kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Interacts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest.

Nynke Y. Rots, Blood, Apr 1999; 93: 2721 - 2729.
Stefan Faderl, Clin. Cancer Res., Jul 1999; 5: 1855.
George Wu, Mol. Biol. Cell, Mar 2007; 18: 1018 - 1029.
A. Koenig, Vet. Pathol., Jul 2002; 39: 458.

• Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using p15 INK antibody #33457.
• Immunofluorescence analysis of HeLa cells, using p15 INK antibody #33457.
• Western blot analysis of extracts from HeLa cells, using p15 INK antibody #33457.
• Western blot analysis of extracts from 293 cells (Lane 2), using p15 INK antiobdy #33457. The lane on the left is treated with synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting