• Product Namep18 INK Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total p18 INK protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from human p18 INK antibody.
• Target Namep18 INK
• Alternative Names    OTTHUMP00000046546; p18(INK4C); p18-INK4C; CDK6 inhibitor p18; cyclin-dependent inhibitor
• Accession No. Swiss-Prot: P42773
  NCBI Gene ID: 1031
• UniprotP42773
• Gene ID1031;
• SDS-PAGE MW 18kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Interacts strongly with CDK6, weakly with CDK4. Inhibits cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB.

Youzhong Yuan, Cancer Res., Jan 2006; 66: 343 - 351.
A. Koenig, Vet. Pathol., Jul 2002; 39: 458.
Pei-Fang Hung, Am J Physiol Cell Physiol, May 2005; 288: C1094 - C1108.
Mana Mirjany, J. Pharmacol. Exp. Ther., May 2002; 301: 494.

• Western blot analysis of extracts from COS7 cells, using p18 INK antibody #33458.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p18 INK antibody #33458.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting