• Product Namep50 Dynamitin Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total p50 dynamitin protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from human p50 dynamitin.
• Target Namep50 Dynamitin
• Alternative Names    50 kDa dynein-associated polypeptide; DCT2; DCTN-50; DCTN2; dynactin complex 50 kDa subunit
• Accession No. Swiss-Prot: Q13561
  NCBI Gene ID: 10540
• UniprotQ13561
• Gene ID10540;
• SDS-PAGE MW 50kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development.

Scott S. Grieshaber, J. Cell Sci., Sep 2003; 116: 3793.
Janis K. Burkhardt, J. Cell Biol., Oct 1997; 139: 469.
Karin A. Melkonian, J. Biol. Chem., Apr 2007; 10.1074.
Lili Yue, J. Biol. Chem., Jul 2000; 275: 23948.

• Western blot analysis of extracts from A549 cells, using p50 dynamitin antibody #33461.
• Western blot analysis of extracts from HuvEc cells (Lane 2), using p50 Dynamitin antiobdy #33461. The lane on the left is treated with synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting